Journal: Molecular cell

Article Title: ZFX mediates non-canonical oncogenic functions of the androgen receptor splice variant 7 (AR-V7) in castrate-resistant prostate cancer

doi: 10.1016/j.molcel.2018.08.029

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SureBeadsTM immunoprecipitation Kit with protein A and G conjugated magnetic beads , Biorad , , 161–4833 , .

Techniques: Immunoprecipitation, Virus, Recombinant, Electron Microscopy, Transfection, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Purification, RNA Library Preparation, Multiplex Assay, Proliferation Assay, Mutagenesis, Magnetic Beads, Western Blot, Stripping, Membrane, shRNA, Knockdown, Control, Construct, Plasmid Preparation, Software

Journal: Cell death and differentiation

Article Title: ARID1A mutations protect follicular lymphoma from FAS-dependent immune surveillance by reducing RUNX3/ETS1-driven FAS-expression.

doi: 10.1038/s41418-025-01445-3

Figure Lengend Snippet: Fig. 3 Identification of the FAS-regulating RUNX3/ETS1 co-transcriptional complex. A Analysis workflow and results of FAS-regulating transcription factors (TFs) and co-transcription factors (co-TFs). B ATAC-Seq differential openness analysis of ARID1AMUT single-cell-derived clones vs ARID1AWT control clones, in OCI-Ly8 (left panel) and OCI-Ly1 (right panel). Heatmap of log10 counts of all detected (open) peaks in the promotor regions of ETS1 and RUNX3 (ARID1AWT (N = 6, in the columns) and ARID1AMUT (N = 9, in the columns)), stars represent statistically significant p-values. C ETS1 gene expression analysis by RNA-Seq in ARID1AMUT clones (blue for het, red for KO) vs ARID1AWT clones (black). D RUNX3 gene expression analysis by RNA-Seq in ARID1AMUT clones (blue for het, red for KO) vs ARID1AWT clones (black). P-values for (C, D) were calculated using the DESeq2 package for differential expression analysis with the default Benjamini-Hochberg correction. Het and KO values were tested together against WT. Pooled data from biological replicates (N) are represented as mean ± SD.

Article Snippet: SureBeadsTM Protein A Magnetic Beads (BioRad) were coupled with anti-ETS1 antibody (CS#14069, Cell Signaling, Danvers, MA, USA) for 3 h at 4 °C with continuous rotation.

Techniques: Derivative Assay, Clone Assay, Control, Gene Expression, RNA Sequencing, Quantitative Proteomics